A new and ecological miniaturized method by spectrophotometry for quantification of vancomycin in dosage form

Vancomycin, an important antibiotic, is marketed as lyophilized powder. In the context of routine analysis this product, existence a more advantageous and effective method interesting. Thus, objective work to develop validate new analytical method, faster, low-cost, ecological miniaturized for quantification vancomycin in powder using spectrophotometry ultraviolet region. Buffer solution pH 6.8, quartz cuvette with capacity 700 µL 280 nm were chosen. The proved be linear range 50-150 µg/mL (0.9997). selectivity was proven two ways: standard-sample overlay aimed identify sample; forced degradation test (sample solutions prepared 0.01 M HCl, NaOH aqueous conditions kept at 60 ºC by 8 hours, UV 254 ambient temperature during 24 hours) show susceptibility consequently indicate stability sample. It precise intraday (RSD 1.27%), interday 1.18%) between analysts 1.92%) levels. robust when small variations performed seven parameters (wavelength, cuvette, filtration step, dibasic monobasic phosphate brand, ultrasound time source water). accuracy standard recovery showed mean 101.10%. This can applied quality control it effective, accessible alternative, which follows Green Analytical Chemistry principles, presenting less waste generation, no use toxic solvents, smaller sample volumes required diluents, impacts on final cost analyzes.